Translocation Transfer

Päivi Tammela

Affiliation:

University of Helsinki,
Finland

Contact:

Phone: +358 50 448 0886
Email: paivi.tammela@helsinki.fi
Website: https://www.helsinki.fi/en/researchgroups/bioactivity-screening

Key words:

Efflux inhibitors, cell-based screens, assay development

Expertise:

Assay development and screening, bioreporters, cell- and target-based assays. The Bioactivity Screening Group at the Faculty of Pharmacy, University of Helsinki, focuses on enhancing antimicrobial drug discovery – covering aspects from early target validation to detailed characterisation studies of novel antimicrobial substances. We are a specialist screening site in EU-OPENSCREEN ERIC and a partner in the JPIAMR project RESET-ME (2019-2022), focused on restoring the E. coli sensitivity for antibiotics by blocking TolC-mediated efflux.

Role in the network:

Development of tools and approaches for antibacterial screening, using both target- and cell-based screening assays, and thus enhancing the discovery of novel antibacterial agents.

References:

• Ilina et al.2018.Miniaturized whole-cell bacterial bioreporter assay for identification of quorum sensing interfering compounds. J. Microbiol. Methods, 154: 40-45.doi: 10.1016/j.mimet.2018.10.005
• Durcik et al.2018.Synthesis and evaluation of N-phenylpyrrolamides as DNA gyrase B inhibitors.ChemMedChem, 13: 186-198.doi: 10.1002/cmdc.201700549